Aceasta este o listă bibliografică construită cu scopul prezentării unei imaginii de ansamblu asupra studiilor populaționale efectuate pe teritoriul României. Lista este împărțită în trei mari secțiuni ce reflectă principalele instrumentele genetice folosite în genetica populațiilor umane.
I. ADN MITOCONDRIAL
| Cocoş R, Schipor S, Hervella M, Cianga P, Popescu R, Bănescu C, Constantinescu M, Martinescu A, Raicu F. Genetic affinities among the historical provinces of Romania and Central Europe as revealed by an mtDNA analysis. BMC Genetics 2017, 18:20. [download]
2016 |Turchi C
, Stanciu F, Paselli G, Buscemi L, Parson W, Tagliabracci A. The mitochondrial DNA makeup of Romanians: A forensic mtDNA control region database and phylogenetic characterization
. Forensic Sci Int Genet. 24 (2016) 136–142.
2014 | Hervella M
, Izagirre N, Alonso S, Ioana M, Netea MG, de-la-Rua C. The Carpathian range represents a weak genetic barrier in South-East Europe. BMC Genetics 2014, 15:56. [download]
2007 | Egyed B
, Brandstätter A, Irwin JA, Pádár Z, Parsons TJ, Parson W. Mitochondrial control region sequence variations in the Hungarian population: analysis of population samples from Hungary and from Transylvania (Romania). Forensic Sci Int Genet. 2007;1(2):158-62.
2006 | Bosch E
, Calafell F, González-Neira A, Flaiz C, et al. Paternal and maternal lineages in the Balkans show a homogeneous landscape over linguistic barriers, except for the isolated Aromuns. Ann Hum Genet. 2006;70(Pt 4):459-87. [download]
2004 | Cardos G
, Stoian V, Miritoiu N, Comsa A, Kroll A, et al. Paleo-mtDNA analysis and population genetic aspects of old Thracian populations from South-East of Romania. Rom J Leg Med. 2004:12(4)239–46. [download]
II. CROMOZOMUL Y
2013 | Varzari A
, Kharkov V, Nikitin AG, Raicu F, Simonova K, et al. Paleo-Balkan and Slavic Contributions to the Genetic Pool of Moldavians: Insightsfrom the Y Chromosome. PLoS ONE 2013;8(1):e53731. [download]
III. ADN NUCLEAR
2013| Stanciu F
, Vladu S, Cutar V, Cocioaba D, Iancu F, et al. Genetic parameters and allele frequencies of five new european standard set STR loci (D10S1248, D22S1045, D2S441, D1S1656, D12S391) in the population of Romania. Croat Med J. 2013;54(3):232-7. [download]
2010 | Demeter SJ
, Kelemen B, Székely G, Popescu O. Genetic variation at 15 polymorphic, autosomal, short tandem repeat loci of two Hungarian populations in Transylvania, Romania. Croat Med J. 2010;51(6):515-523. [download]
2007 | Marian C
, Anghel A, Bel SM, Ferencz BK, Ursoniu S, et al. STR data for the 15 AmpFlSTR identifiler loci in the Western Romanian population. Forensic Sci Int. 2007;170(1):73-5.
Barbarii LE, Rolf B, Constantinescu C, Hohoff C, et al. Allele frequencies of 13 short tandem repeat (STR) loci in the Romanian population. Forensic Sci Int. 2004;141:171–174.
2003 | Anghel A
, Marian C, Pitulescu M, Daba A, Sirbu IO, et al. Population genetic study of eight short tandem repeat loci CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, F13B and LPL in the Western Romanian population. Forensic Sci Int. 2003;131:218–219.
2003 | Anghel A
, Motoc M, Marian C, Motoc A, Neagu M, Gaili MA, et al. Genetic Differences in Western Romanian Population Between Autochthonous Caucasian Population and an African Origin Subpopulation Group. Timisoara Med J 2003;1. [download]
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27-30 September 2011 10th International Symposium on Forensic Sciences, Bratislava, Slovakia
Building a minimalist DNA database management system using Pruem tools (poster)
When it comes of DNA data exchange base on 2008/615/JHA and 2008/616/JHA Council Decisions, each EU country is free of choosing the implementation means. Romania has some experience in implementing three different types of IT solutions: a bought one – Dimensions 2.0, an in-house built solution – Pruem tools and a freeware solution – CODIS 7.0, each of them with their specific advantages and disadvantages. Starting with the Pruem DNA data exchange infrastructure and base on Pruem tools any DNA database laboratory, which has some Pruem DNA data exchange experience, can build from scratch a simple DNA database management system for laboratory internal DNA profiles comparison purposes, with a minimum of human and IT resources. This paper presents a review of the Romanian National DNA Database Laboratory efforts in building a minimalist DNA database management system using the most common and freely available Pruem tools: Communication component (Germany), Match engine (Austria) and Graphical user interface (Netherlands).
Keywords: Pruem DNA data exchange, Pruem tools, DNA database management system, Romanian National DNA Database Laboratory.
18-20 September 2008 – 4th Medical Genetic Romanian Association Conference, Craiova, Romania.
a) Stanciu F, Sevastre O, Tinischi M, Stoian V.
Valorificarea reziduurilor biologice obţinute in urma analizelor medicale pentru studii de Genetica Forensica (poster)
În situații deosebite în care recoltarea de probe biologice de referință este imposibilă prin metodele clasice, analizele medicale efectuate în laboratoarele de screening, respectiv reziduurile biologice obținute în urma efectuării acestora, pot furniza date genetice valoroase cu aplicabilitate in genetica forensică. În acest sens am evaluat o serie de reziduuri in componența cărora pot exista celule nucleate pretabile genotipării, urmărind factori precum timpul și condițiile de păstrare ale probelor biologice în laboratoarele medicale, degradarea ADN-ului, prezența contaminării, rata succesului calculata la numărul de loci amplificaţi, etc.. Am efectuat izolarea totală a ADN-ului folosind o forma adaptata a protocolului AGOWA mag DNA Isolation Kit pentru platforma automată de manipulare a lichidelor Freedom EVO 150. Din extractele de ADN obținute au fost amplificați 16 markeri STR folosind AmpFlSTR Identifiler PCR Amplification Kit. Ampliconii au fost analizați prin electroforeza capilară folosind analizorul ABIPrism 3100. Rezultatele obținute au fost interpretate folosind GeneScan și Genotyper Software conform standardelor specifice analizelor de genetica forensică.
b) Duta-Cornescu G, Stoian V, Constantin N, Simon-Gruita A, Stanciu F, Banica D, Tuduce R, Cristea P.
Genetic Studies about TB Susceptibility in Romanian Population (poster)
25-26 April 2008 – Forensica 2008, Praque, Czech Republic.
a) Stanciu F, Stoian IM, Popescu OR.
Evaluation of Freedom Evo 150 with AGOWA sep9600 for Databasing Purposes (poster)
Automation is crucial nowadays in databasing laboratories especially in those countries which are relative new in the field and must quickly reach the minimum standards of consecrated databasing laboratories from Europe and USA. In our efforts to build Romanian National DNA Database we evaluated the Freedom Evo 150 Liquid Handling Platform with Agowa 9600 Magnetic Separator in different running conditions and flux implementations, for reaching the most efficient ones. We compared the results with those obtained from manual extraction methods such as magnetic DNA extraction and Chelex DNA extraction. For this we used a total of 6246 biological reference samples (5220 – automated DNA extraction and 1026 – manually DNA extraction), which we amplified using AmpFlstr Identifiler Kit. The obtained PCR products had been analyzed by capillary electrophoresis using ABI Prism 3100 Genetic Analyzer.
Keywords: automation, databasing laboratory, liquid handling platform, DNA magnetic separator
b) Stoian MG, Galan E, Stanciu F, Ciofu V.
Comparative Study of Soils from Several Romanian South-East Regions and their Influence on DNA Degradation in Tooth Samples, From Different Periods of Time (poster)
The degradation process of tooth and bone tissues and also of the DNA from them is a very complex one, determined by many concurrent factors which can be classified in three main categories – biological, physical and chemical factors. This paper presents our research regarding morphological aspects of soils and the influence of chemical composition on DNA degradation through fossilization process. In order to achieve our goals, we collected 27 soil samples from different archaeological sites, from various depths and different archeologically periods of time – Neolithic, The Bronze Age, The First Iron Age, The Romano-Byzantine Age, VIII-X century. We performed pH determination, calcination losses, SEM/EDS analysis of soil samples and we correlated our results with the DNA degradation index obtained from fossilized tooth samples collected from the same locations as soils. The results obtained from this analysis represent a small part of our efforts in reaching out the main objectives: a) establishing the identification and differentiation criteria for forensic soils; b) establishing which types of soil better preserves the human DNA from teeth and bones for further genetic analyses.
Keywords: Soil, Fossilization, Teeth, DNA degradation index
[visit the official website]
03-07 September 2007 – 5th ISABS Conference in Forensic Genetics and Molecular Anthropology, Split, Croatia.
a) Stanciu F, Potorac R, Veronica Stoian V.
Indirect approach in finding someone’s autosomal genetic profile – a forensic tool in investigating historical cases (poster)
b) Stanciu F, Galan E, Stoian MG, Andreescu G, Soficaru AD, Miriţoi N.
Comparative study of several tooth samples from different archaeological sites and the implications of fossilization in preservation of DNA overtime (poster)
[visit the official website]